A1 HYDROGEL
OVERVIEW
SunP Gel A1 is a biodegradable bio-ink developed for 3D bioprinting. It has good biocompatibility and fast degradation making it suitable for most human and animal-derived cells. The cell survival rate after printing is higher than 85%, and the product comes in both gel & powder form
ADVANTAGES
- Good biocompatibility, degradable
- Good molding effect and long structure retention time (more than 30 days)
- Easy to operate, mix with cell suspension to print
- Wide range of applications, suitable for most human and animal cells
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TECHNICAL SPECIFICATIONS
RHEOLOGICAL PROPERTIES
A1 GEL
A1 POWDER
The gel temperature was measured by Anton Paar MCR302 rotational rheometer. Measurement parameters: deformation range 0.1%, test frequency 1 Hz, cone plate diameter 25 mm, cone plate slope 2 °, cone plate The distance between the tip of the cone and the bottom plate is 99 μm. Gel point test results: 24.0-26.0 °C
CELL VIABILITY STUDIES
A1 GEL
A1 POWDER
Figure 3: HUVEC-24h live staining
The cell viability of Hela, A549, NIH-3T3 and HUVEC cells was detected by Calcein AM-PI kit for 24 hours. Under confocal observation, the living cells showed green fluorescence and the dead cells showed red fluorescence (Figure 3).
CELL VIABILITY TEST RESULTS
| Cell type | Hela | A549 | NIH-3T3 | HUVEC |
| Survival rate | ≥90% | ≥85% | ≥90% | ≥90% |
Figure 4: HUVEC-24h live staining
The cell viability of Hela, A549, NIH-3T3 and HUVEC cells was detected by Calcein AM-PI kit for 24 hours. Under the confocal observation, the living cells showed green fluorescence and the dead cells were red. Fluorescence (Figure 4).
CELL VIABILITY TEST RESULTS
| Cell type | Hela | A549 | NIH-3T3 | HUVEC |
| Survival rate | ≥90% | ≥90% | ≥85% | ≥90% |
PRINTING APPLICATION EXAMPLE
Bio-ink was prepared by mixing NIH-3T3 cell suspension with SunP Gel A1, and the three-dimensional structure was printed. The culture medium was cultured for 7 days, and the cells were proliferated from single cells into cell clusters. The specific morphology is shown in Figure 5.
Figure 5: NIH-3T3 cell mass (7day)
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