G1 HYDROGEL
OVERVIEW
SunP Gel G1 is a bioInk based on methacrylate gelatin (Gel-MA), which retains the temperature-sensitive properties of gelatin and can be mixed with photoinitiators (such as LAP, I2959). It crosslinks under blue light or ultraviolet light, and suitable for most human and animal-derived cells. The product is available in gel and powder form
ADVANTAGES
- Good biocompatibility, cell survival rate is higher than 90%
- Good molding effect and long structure retention time (more than 30 days)
- Easy to operate, mix with cell suspension to print
- Wide range of applications, suitable for most human and animal cells
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TECHNICAL SPECIFICATIONS
RHEOLOGICAL PROPERTIES
Figure 1: Gel point detection method
The gel temperature was measured by Anton Paar MCR302 rotational rheometer. The measurement parameters were: deformation range 0.1%, test frequency 1 Hz, cone plate diameter 25 mm, and the slope of the cone plate was 2°, the cone tip of the tapered plate is 99 μm away from the bottom plate. Gel point test results: 23.0-25.0 °C
CELL VIABILITY STUDY
Figure 2: HUVEC-24h live staining
Cell Viability Detection method: The cells of Hela, A549, NIH-3T3 and HUVEC were tested for 24-hour cell viability by Calcein AM-PI kit. Under confocal observation, the living cells showed green fluorescence and the dead cells showed red fluorescence (Figure 2 And head video), cell viability test results:
| Cell type | Hela | A549 | NIH-3T3 | HUVEC |
| Cell viability | ≥90% | ≥90% | ≥85% | ≥90% |
TYPICAL APPLICATIONS
SunP Gel G1 mixed HUVEC cell suspension, preparation of bio-ink, printing of three-dimensional structure, normal culture for 7 days, cells from a single The cells proliferate and connect into a network, as shown in Figure 3.
Figure 3 HUVEC morphology staining
(a: brightfield photos, b: DAPI stained nuclei, c: F-actin, d: ICAM1, e: merge)
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